Análisis in vitro de espermatozoides tras exposición a herbicida y nicotina. Efecto antioxidante y protector de la melatonina
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2022-07-11
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Jaén: Universidad de Jaén
Resumen
El glifosato, herbicida más utilizado en el mundo en la agricultura y la nicotina, uno de los componentes principales del tabaco, se han vuelto preocupantes para la salud reproductiva de la humanidad. Este trabajo tiene como objetivo comprobar el efecto de estos dos componentes en el esperma porcino y los posibles efectos protectores de un antioxidante, la melatonina. Para ello las muestras seminales se dividieron en 6 grupos experimentales. En las alícuotas se analizaron diferentes variables: vitalidad, apoptosis espermática, generación de especies reactivas de oxígeno (ROS), daños de membrana y fragmentación de ADN. Los resultados mostraron un aumento de la mortalidad, un mayor número de especies reactivas de oxígeno y apoptosis, por ambas sustancias dañinas para nuestro organismo. También se observó que aumentaban los daños de membrana, así como daños en el ADN, ambas solo por el glifosato. Por último, la melatonina ejerció un gran poder protector frente al glifosato cuanto a la vitalidad y la apoptosis espermática, en los demás no se observó una mejora significativa.
Glyphosate, the most widely used herbicide in agriculture in the world, and nicotine, one of the main components of tobacco, have become a concern for the reproductive health of humanity. The objective of this work is to verify the effect of these two components in pig sperm and the possible protective effects of an antioxidant, melatonin. For this, the seminal samples were divided into 6 experimental groups. Different variables were analysed in the aliquots: vitality, sperm apoptosis, generation of reactive oxygen species (ROS), membrane damage and DNA fragmentation. The results showed an increase in mortality, a greater number of reactive oxygen species and apoptosis, due to both substances that are harmful to our body. Membrane damage, as well as DNA damage, were also observed to be increased, both by glyphosate alone. Finally, melatonin exerted a great protective power against glyphosate in terms of sperm vitality and apoptosis, in the others no significant improvement was observed.
Glyphosate, the most widely used herbicide in agriculture in the world, and nicotine, one of the main components of tobacco, have become a concern for the reproductive health of humanity. The objective of this work is to verify the effect of these two components in pig sperm and the possible protective effects of an antioxidant, melatonin. For this, the seminal samples were divided into 6 experimental groups. Different variables were analysed in the aliquots: vitality, sperm apoptosis, generation of reactive oxygen species (ROS), membrane damage and DNA fragmentation. The results showed an increase in mortality, a greater number of reactive oxygen species and apoptosis, due to both substances that are harmful to our body. Membrane damage, as well as DNA damage, were also observed to be increased, both by glyphosate alone. Finally, melatonin exerted a great protective power against glyphosate in terms of sperm vitality and apoptosis, in the others no significant improvement was observed.