Impacto del cigarrillo electrónico en la viabilidad de los espermatozoides. Estudio In vitro del daño de membrana y generación de especies ROS
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2023-07-04
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Jaén: Universidad de Jaén
Resumen
Según la Organización Mundial de la Salud, los casos de infertilidad masculina han aumentado en la última década. En 1999, la concentración promedio de espermatozoides era de 20 millones por mililitro, pero en 2010 había disminuido a 15 millones. Fumar afecta negativamente la calidad del esperma, y aunque los cigarrillos electrónicos se consideran más seguros que el tabaco, el líquido de recarga que contienen contiene componentes que perjudican la producción de espermatozoides. Estos componentes incluyen nicotina, propilenglicol, glicerina y saborizantes, que pueden ser tóxicos para los espermatozoides. En este estudio se examinó los efectos a corto plazo del líquido de recarga en los espermatozoides. Se estableció un grupo de control y cuatro grupos experimentales que fueron expuestos a nicotina pura y a E-Liquid con nicotina durante diferentes períodos y concentraciones. Se midió la vitalidad, el daño de la membrana, las especies reactivas de oxígeno y la apoptosis. Los resultados mostraron que una exposición más prolongada y concentraciones más altas llevaron a una disminución en la vitalidad y un aumento en las especies reactivas de oxígeno. Sin embargo, no se encontraron hallazgos significativos en cuanto al daño de la membrana y la apoptosis.
According to the World Health Organization, cases of male infertility have increased in the last decade. In 1999, the average sperm concentration was 20 million per milliliter, but by 2010, it had decreased to 15 million. Smoking negatively affects sperm quality, and although electronic cigarettes are considered safer than tobacco, the refill liquid they contain includes components that harm sperm production. These components include nicotine, propylene glycol, glycerin, and flavorings, which can be toxic to sperm. This study examined the short-term effects of the refill liquid on sperm. A control group and four experimental groups were exposed to pure nicotine and E-Liquid with nicotine for different periods and concentrations. Vitality, membrane damage, reactive oxygen species, and apoptosis were measured. The results showed that longer exposure and higher concentrations led to a decrease in vitality and an increase in reactive oxygen species. However, no significant findings were found regarding membrane damage and apoptosis.
According to the World Health Organization, cases of male infertility have increased in the last decade. In 1999, the average sperm concentration was 20 million per milliliter, but by 2010, it had decreased to 15 million. Smoking negatively affects sperm quality, and although electronic cigarettes are considered safer than tobacco, the refill liquid they contain includes components that harm sperm production. These components include nicotine, propylene glycol, glycerin, and flavorings, which can be toxic to sperm. This study examined the short-term effects of the refill liquid on sperm. A control group and four experimental groups were exposed to pure nicotine and E-Liquid with nicotine for different periods and concentrations. Vitality, membrane damage, reactive oxygen species, and apoptosis were measured. The results showed that longer exposure and higher concentrations led to a decrease in vitality and an increase in reactive oxygen species. However, no significant findings were found regarding membrane damage and apoptosis.