Estudio del metabolismo de la sucralosa en orina
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2014-09-12
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Jaén: Universidad de Jaén
Resumen
La sucralosa es un edulcorante no nutritivo y es aproximadamente 600 veces más
dulce que el azúcar de mesa. Se utiliza en un amplio rango de comidas y bebidas. En este trabajo, se desarrolló una metodología para la separación cromatográfica y el análisis de la sucralosa en muestras de orina empleando cromatografía líquida/tiempo de vueloespectrometría de masas (LC/TOF-MS). El edulcorante se extrajo de las muestras de orina empleando extracción en fase sólida (SPE) con cartuchos poliméricos (PLEXA). La presencia del edulcorante se confirmó mediante medidas de masa exacta de la molécula desprotonada [M-H]- y sus aductos de sodio y cloro. Para estimar la cantidad de sucralosa ingerida y el porcentaje de sucralosa excretada, la cuantificación se llevó a cabo usando calibración estándar con ajuste matricial. Por último, el análisis de estas muestras de orina confirmó que la sucralosa no se metaboliza fácilmente en el cuerpo y sólo una pequeña cantidad (aprox. 15%) se excreta inalterada en la orina. Además, la sucralosa sufre conjugación glucurónica (confirmado con la identificación del correspondiente metabolito en la orina).
Sucralose is a non-nutritive sweetener and is approximately 600 times sweeter than table sugar. It is used in a wide range of foods and beverages. In this study, a methodology was developed for the chromatographic separation and analysis of sucralose in urine samples using liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS). The sweetener was extracted from urine samples using solid-phase extraction (SPE) with polymeric cartridges (PLEXA). The presence of the sweetener was confirmed by exact mass measurements of the deprotonated molecule [M-H]- and its sodium and chloride adducts. To estimate the amount of sucralose ingested and the percentage of sucralose excreted, quantification was performed using standard calibration with matrix matching. Finally, analysis of these urine samples confirmed that sucralose is not readily metabolized in the body, and only a small amount (approximately 15%) is excreted unchanged in the urine. Furthermore, sucralose undergoes glucuronide conjugation (confirmed by the identification of the corresponding metabolite in the urine).
Sucralose is a non-nutritive sweetener and is approximately 600 times sweeter than table sugar. It is used in a wide range of foods and beverages. In this study, a methodology was developed for the chromatographic separation and analysis of sucralose in urine samples using liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS). The sweetener was extracted from urine samples using solid-phase extraction (SPE) with polymeric cartridges (PLEXA). The presence of the sweetener was confirmed by exact mass measurements of the deprotonated molecule [M-H]- and its sodium and chloride adducts. To estimate the amount of sucralose ingested and the percentage of sucralose excreted, quantification was performed using standard calibration with matrix matching. Finally, analysis of these urine samples confirmed that sucralose is not readily metabolized in the body, and only a small amount (approximately 15%) is excreted unchanged in the urine. Furthermore, sucralose undergoes glucuronide conjugation (confirmed by the identification of the corresponding metabolite in the urine).